(isoleucine)
Enzyme 5
Intermediate D
Intermediate B
Intermediate A
Enzyme 4
Enzyme 2
Enzyme 3
Initial substrate
(threonine)
Threonine
in active site
Active site
available
Active site of
enzyme 1 no
longer binds
threonine;
pathway is
switched off.
Isoleucine
binds to
allosteric
site
Slide 76
Structures within the cell help bring order to metabolic pathways
Some enzymes act as structural components of membranes
In eukaryotic cells, some enzymes reside in specific organelles; for example, enzymes for cellular respiration are located in mitochondria
Slide 77
Fig. 8-23
1 µm
Mitochondria
Slide 78
Fig. 8-UN2
Progress of the reaction
Products
Reactants
∆G is unaffected
by enzyme
Course of
reaction
without
enzyme
Free energy
EA
without
enzyme
EA with
enzyme
is lower
Course of
reaction
with enzyme
Slide 79
Fig. 8-UN3
Slide 80
Fig. 8-UN4
Slide 81
Fig. 8-UN5
Slide 82
Distinguish between the following pairs of terms: catabolic and anabolic pathways; kinetic and potential energy; open and closed systems; exergonic and endergonic reactions
In your own words, explain the second law of thermodynamics and explain why it is not violated by living organisms
Explain in general terms how cells obtain the energy to do cellular work
Slide 83
Explain how ATP performs cellular work
Explain why an investment of activation energy is necessary to initiate a spontaneous reaction
Describe the mechanisms by which enzymes lower activation energy
Describe how allosteric regulators may inhibit or stimulate the activity of an enzyme