Cytoskeletal track
Motor protein
Protein moved
(a) Transport work: ATP phosphorylates
transport proteins
ATP
ATP
Slide 45
ATP is a renewable resource that is regenerated by addition of a phosphate group to adenosine diphosphate (ADP)
The energy to phosphorylate ADP comes from catabolic reactions in the cell
The chemical potential energy temporarily stored in ATP drives most cellular work
Slide 46
Fig. 8-12
P
i
ADP
+
Energy from
catabolism (exergonic,
energy-releasing
processes)
Energy for cellular
work (endergonic,
energy-consuming
processes)
ATP
+
H2O
Slide 47
Concept 8.4: Enzymes speed up metabolic reactions by lowering energy barriers
A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction
An enzyme is a catalytic protein
Hydrolysis of sucrose by the enzyme sucrase is an example of an enzyme-catalyzed reaction
Slide 48
Fig. 8-13
Sucrose (C12H22O11)
Glucose (C6H12O6)
Fructose (C6H12O6)
Sucrase
Slide 49
Every chemical reaction between molecules involves bond breaking and bond forming
The initial energy needed to start a chemical reaction is called the free energy of activation, or activation energy (EA)
Activation energy is often supplied in the form of heat from the surroundings
Slide 50
Fig. 8-14
Progress of the reaction
Products
Reactants
∆G < O
Transition state
Free energy
EA
D
C
B
A
D
D
C
C
B
B
A
A
Slide 51
Enzymes catalyze reactions by lowering the EA barrier
Enzymes do not affect the change in free energy (∆G); instead, they hasten reactions that would occur eventually
Animation: How Enzymes Work
Slide 52
Fig. 8-15
Progress of the reaction
Products
Reactants
∆G is unaffected
by enzyme
Course of
reaction
without
enzyme
Free energy
EA
without
enzyme
EA with
enzyme
is lower
Course of
reaction
with enzyme
Slide 53