Cell And Tissue Culture

Page
2

Proliferation can begin again

Slide 15

2 main categories of animal cell cultures….

Primary culture

Continuous cell line

Slide 16

Primary Cultures

Taken from fresh tissue

Limited life span in culture

Treated by proteolytic enzyme (Trypsin)

Separate into single cells

-epithelial cells

-fibroblasts

Slide 17

Continuous Cell Line

Derived from humans

Been transformed

-lose sensitivity to factors associated with growth control

Produce immortalised cell lines

Cell lines are neoplastic

Often lose their anchorage-dependence

-associated with an altered xsome pattern

More easily cultured

Slide 18

Slide 19

Why do it?

Particular cells can be isolated and cloned

-isolation of mutant cells

-investigate cell growth

-produce hybrid cells that have applications in biotechnology

Produce important pharmaceuticals

-vaccines

-hormones