Real time PCR and it's functions in diagnosis

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Some peptides which are classified in an antimicrobial group , can have an effect on immune system ,for example Intercellular adhesion molecule-1(ICAM-1) is expressed at a low level in a subpopulation of haematopoietic cells, vascular endothelium, fibroblasts and epithelial cells. However, its expression is dramatically increased at sites of inflammation, providing an important means of regulating cell–cell interaction and thereby inflammatory responses.

Increase in Expression of 1(ICAM-1) will be lowered due to the effect of antimicrobial peptide & this can be detected by Real time PCR using SYBR Green.(12)

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Real time PCR in forensic medicine

Introduction

Advantage disadvantages

Product Detection

Conclusion

Reference

Real Time PCR

in medicine

The main task in The Forensic Medicine is to investigate deaths from unnatural causes.

Forensic science has embraced the use of DNA molecular biology tools for diagnostic purposes more than any other scientific field.

The process of routine forensic human identification involves sensitive PCR and can be performed successfully on most evidence materials found at a crime scene.

This real-time technology monitors the accumulation

of PCR product with each cycle and allows assessment of each sample individually during the exponential growth phase.quantification assay has proven to be highly sensitive,specific,rapid, cost-effective and flexible assay for analysis of forensic casework samples.

REAL TIME PCR & IT’S FUNCTIONS IN DIAGNOSIS

DNA tests performed by a U.S. laboratory have proved that bone fragments exhumed in the Ural Mountains in 2007 belong to two children of Russia's last czar.

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Some kites to detection of pathogens by Real Time PCR

Adenoviruses different type

Bordetella pertussis

Herpsvirus (Bovine & Human all type )

Leukemia virus

Klebsiella pneumoniae

Influenza A virus

Helicobacter pylori

Hepatitis virus ( A,B,C,Delta )

HIV ( type1&2 )

Leishmania

Neisseria meningitidis

Respiratory Syncytial virus

Shigella

Streptococcus

& …

Introduction

Advantage disadvantages

Real Time PCR

Product Detection

in medicine

Conclusion

Reference

REAL TIME PCR & IT’S FUNCTIONS IN DIAGNOSIS

Slide 30

Conclusion

Introduction

Advantage disadvantages

Real Time PCR

Product Detection

in medicine

Conclusion

Reference

REAL TIME PCR & IT’S FUNCTIONS IN DIAGNOSIS

PCR has proved to be a useful tool in research and diagnosis. However, its use has also brought new challenges to research. The sensitivity found in PCR technology and the availability of quantitative results will bring new problems to the interpretation of these results. A great deal of work is needed to generate a basis of knowledge for correct interpretation of these tests. In medicine, PCR-based diagnostics are just becoming widely used and because of the increased cost-effectiveness of the newer assays, knowledge for their interpretation will soon become available.