Optics - Filter Properties & manipulation of light in flow cytometry

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Slide 38

Fiber optics & optical waveguides

Fiber optics and other optical waveguides operate by total internal reflection

problems with stray light, low NA of fibers, thus low sensitivity, light collection difficult

µ

cladding

Fiber optic waveguide

Slide 39

Optics for forward scatter

scatter

detector

iris

blocker

Laser beam

Stream in air or a round capillary

Slide 40

Lecture Summary

At the conclusion of this lecture the student should understand:

Field stops and obscuration bars are necessary in systems where air or round capillaries are used

Appropriate optical filters must be placed in combinations

Filters degrade over time and should be checked

The least number of filters should be used in a system

Forward angle scatter is frequently collected using a diode detector