Pasteur's Experiment - Step 1
S-shaped Flask
Filled with broth
The special shaped was intended to trap any dust particles containing bacteria
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Pasteur's Experiment - Step 2
Flasks boiled
Microbes Killed
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Pasteur's Experiment - Step 3
Flask left at various locations
Did not turn cloudy
Microbes not found
Notice the dust that collected in the neck of the flask
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Pasteur's Experimental Results
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Pasteur’s S-shaped flask kept microbes out but let air in.
Proved microbes only come from other microbes (life from life) - biogenesis
The Theory of Biogenesis
Figure 1.3
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1668: Francisco Redi filled six jars with decaying meat.
Evidence Pro and Con
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1745: John Needham put boiled nutrient broth into covered flasks.
Evidence Pro and Con
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1765: Lazzaro Spallanzani boiled nutrient solutions in flasks.
Evidence Pro and Con
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1861: Louis Pasteur demonstrated that microorganisms are present in the air.
Evidence Pro and Con
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