Lab Respiration

Page
4

Tube 4: Add 5 ml of water to the test tube instead of liquid hamburger. Then add 10 drops of succinic acid and 3 drops of methylene blue to the hamburger and mix. Note the time as before and watch for the color to change.

Suggestions: Rinse the mixing rod between tubes.

Mix all the tubes at the same time. Do wait for tube 1 to finish reacting before starting tube 2, tube 3, or tube 4.

Slide 15

CELL RESPIRATION

Experimental procedure for The Krebs Cycle and the Action of Succinic (Acid) Dehydrogenase (cont.).

Results: Three of the four tubes should clear. The time required for each of the three tubes should be different. To determine why the times are different you should consider the only variable used in this part of the experiment. One of the tubes should not change. Can you guess which one and why? Your results should look like this picture.